Plants evolved a specific subnuclear "switch" that completely flips how they respond to their most important growth hormone.
Auxin is the hormone that tells a plant how to grow, but the way it works has been a mystery for decades. Researchers found that the Mediator complex clusters into specific groups inside the nucleus to turn repressor genes into activators. This tiny biochemical reorganization is what allows a plant to suddenly change its growth strategy in response to its environment. It is the first time the exact mechanism for this fundamental transition has been identified at the subnuclear level. This discovery explains how plants manage such complex development with a relatively simple set of hormones.
Subnuclear cofactor partitioning underlies auxin-dependent transcriptional regulation
bioRxiv · 10.64898/2026.04.29.721720
Cellular and organismal function relies on the precise activation and repression of gene expression by DNA-binding transcription factors (TFs). Many TFs occur in large gene families, and a key question in biology is how divergent functions emerge in TF families during evolution. Here we discover the biochemical mechanism for transcriptional activation by the Marchantia polymorpha AUXIN RESPONSE FACTOR1 (MpARF1) TF, which relies on direct recruitment of the Mediator complex into subnuclear MpARF1